Cell Lysis of BL21 Cells by Ultrasonication Hielscher Ultrasonics
The protocol below gives a stepbystep direction for ultrasonic BL21 cell lysis: In order to remove the chaperone proteins, BL21 bacterial pellets were resuspended in 50 ml of ice cold Sodium TrisEDTA (STE) buffer (consisting in 10 mM TrisHCL, pH, 1 mM EDTA, 150 mM NaCl supplemented with 100 mM PMSF). The, 500 ul of lysozyme (10 mg/ ml ...